Protective effect of Zizphus vulgaris extract, on liver toxicity induced by carbon tetrachloride in laboratory rats

Sohail Ashkani (
Shiraz Medical School, Shiraz University of medical sciences
June, 2009


Introduction: A large range of human diseases is assigned to liver. Although some of the causes of these diseases are not clear but free radicals are of main factors creating these disorders.
There are Chemical drugs for treatment of various disorders and reduce complications but they have some unwanted side effects. Zizphus vulgaris, from past, was used as a pharmaceutical plant in the East Asia for treatment of liver disorders. This study investigates the protective effect of Zizphus vulgaris extract, on induced liver toxicity with carbon-tetrachloride in rats and effects of Z.Vulgaris extract on ALP, ALT, AST enzymes and also on the total protein, albumin, serum bilirubin, determining the most appropriate dose of Z.Vulgaris extract used in liver damages and the protective effect of Z.Vulgaris extract on liver damage in different doses.

Materials &Methods: This study was done over randomly selected 30 healthy adult male rats, divided into five groups of 6 individuals:
1. Control group: the olive-oil was injected intraperitoneally.
2. Control group: the carbon-tetrachloride was injected intraperitoneally.
3. Test groups III, II, I intraperitoneal injection of carbon tetrachloride and oral ingestion of Chamomile extract in doses of 200, 400 and 600 mg per kg mice were performed. After 45 days, blood sampling was done and serums were sent to the laboratory for review.

Results: The concentration of total protein, albumin, AST, ALT, ALP in test groups I, II and III receiving Z.Vulgaris extract daily (200 doses, respectively, 400 and 600 mg per kg weight) compared with control group was insignificant due to Statistical analysis. Consumption of Z.Vulgaris reduced the bilirubin concentration in test groups I and II. Decrease only in the test group I is significant. Increased Z.Vulgaris dose in the test group III (600 mg Z.Vulgaris per kg body weight) shows increased bilirubin level compared to control group.
Weights in all groups during the test rose, especially in the test group II in comparison to controls that difference was significant, but other factors are insignificant. (P> 0.05)
Ratio of liver weight percentage to body weight of rats in groups I and III to the control group shows increase, although not significant.
Conclusion: This study demonstrates failure of protective effect of jujube plant on liver.

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